day 3:Result

OUR GROUP RESULT

table for the OD and glucose values..

figure 1 : The Graph OD reading at the abs 600nm  againts time

Based on the graph ,its shown the growth of Saccharomyces Cerevisiae.the beginning of this graph is represent the lag phase,at this stage lag phase is not longer. why lag phase is not longer? Because it is not much adaptation to the cell,we already used the same media to inoculate them, so that they don’t have any longer time to adapt with new environment.

After that the graph curve show the growth rapidly, this is due to the growth of Saccharomyces Cerevisiae that have consumed the glucose supply in the fermenter.

The graph curve after that shown the decreasing, this can tell us that Saccharomyces Cerevisiae consumed the glucose and the glucose level in the fermenter was decreasing, so that the curve also decrease, because  most of the cell was die. The consumption of glucose at this stage is at maximum level. so the cell need to competed among themselves.

The amount of glucose become limited, the growth will be decreasing, because of more cell tend to die and only the cell that survive will competed the consumptions of glucose.

However if we supply the glucose at this stage, we see the curve will rapidly increase again.in the fermenter. The temperature was increase because cells themselves also produce heat and to check the contamination of our culture growth ,we may check under the microscope,wheather there are contamination or not.



GRAPH OF THE BIOREACTOR  (Iris)

The fermenter is connected to the window, IRIS.List of available process variable is shown from which individual parameter. Different bioreactor can have different group of parameter associated with them according to the specification.

The on- line graph shows the actual data in real time.OD and a different screen opens to that shown for measured parameters. Oxygen are the most important thing to growth the cell, as oxygen is being consumed by the yeast, oxygen level become decreasing, so that the agitation will be faster to supply enough oxygen to the cell. So that the yeast would not die.

The line for the temperature would also increase because of cell itself produce heat. A red time line can be moved along ,wherever the line intersects the graph data point the value for each parameter at that time are displayed. The bar graph will be changed accordingly

its shown how the system run : fermenter direcly connected with the software