Day 1: Its all begin from this day ...

DAY 1......

Precisely at 1 o'clock, we walked to the room 148... Our lovely laboratory... When we reach the room.. There was already one bioreactor being install by the coordinator... The bioreactor we used is the 2 liters bioreactor imported by INFORS HT, Switzerland...

The bioreactor that already install by the coordinator before we came to the laboratory...

While waiting for the coordinator.. We take our time to take a look the bioreactor.. Compared it from what we learn from the theory classes...

This is our cool coordinator.. Mr.Shaman Gaspar.. He were with us for the whole week teaching us on how to make used of the bioreactor correctly...

Mr.Shaman is giving some information about the INFORS HT company and the bioreactor itself...

The very first thing we need to do that day.. Is unpack the box of the bioreactor.. Take out the bioreactor and put all the components onto the bioreactor...

What is bioreactor/ fermenter???
Bioreactor function is to provide cells and microorganisms cultivated in it with the most optimal condition for vital capacity...

What is the function each of the components in the bioreactor???

2 LITERS BIOREACTOR...

Syringe: To suck out the sample from the vessel...

Vessel holder: To hold the vessel and reagent bottles (min 2,max 4)

Antifoam reagent: Prevent formation of roaming during fermentation

Base reagent: Use to raise up the pH when it too low (acidic)

BASE UNIT...

Operating panel: Menu button to choose all the parameter needed...

Temperature line: To take the temperature in the vessel...

pH line: Connect to pH probe...

pO2 line: Connect to O2 probe...

Antifoam line:

1. Black-to the vessel...
2. Red -to the antifoam probe...

Motor: Supply the power to rotate the impeller movement...

*The motor is located within the base unit... The connection to the vessel is simply lifted for removal and replaced for operation...

BACK OF THE BASE UNIT...

OPERATING PANEL...

Vessel holder: To support the vessel and reagent bottles...

Reagent bottle: Use for bases,acid,feed and antifoam.. But for our experiment we only use two bottle reagent for base and antifoam... we do not need feed reagent bottle because we only do a batch fermentation... We do not use acid during the fermentation because the yeast (Saccharomyces Cerevisiae) itself producing acid...

Rotameter: Manually controlling the amount of air entering the vessel... can be used from 0 l/min to 5 l/min...

Air compressor: Air supply...

SAMPLE BOTTLE...

Sample bottle: Collect the sample during fermentation to make a test...

HEAD PLATE...

Head plate:

1. To get information out of the vessel (pH,O2,temperature...)
2. To get material into or out the vessel (acid,base,nutrients,sample...)

COMPONENTS IN VESSEL..

Sparger:

1. Gases for the maintenance of dissolved oxygen... 
2. Introducing pressurized gas to the fluid through a sparger, which usually have small holes that break the incoming gas into fines bubbles...

Impeller:

1. Six-blade disc impeller--->the type of impeller in our bioreactor...
2. To diminish the size of air bubble to give a bigger inter-facial area for oxygen transfer and to decrease the diffusion path...
3. To maintain a uniform environment throughout the vessel contents...

CLOSE UP OF THE VESSEL

Vessel: Non-jacketed glass vessel place for media...

Baffles:

1. To prevent liquid swirl (vortex) and thereby enable the impeller to impose power on the liquid in the form of turbulence and flow...
2. To improve aeration efficiency...

Harvest line: To harvest the product...

CONDENSER...

Condenser: To cool the liquid in the air outlet resulting in the separation of the foam and other liquid particles..

Exit gas cooler with filter: Sterile the air before going out to the environment...

Inlet filter connected to the Sparger: Sterile air enter the bioreactor...

PROBES...

pH probe & O2 probe: Both probes used to control and maintain the pH and dissolved oxygen parameters at the optimal level...


Then, the last thing we need to do in the first day is to prepare 200ml media for the seed culture...
The ingredient use in the YPEG media are:

1. Yeast Extract 1%
2. Glucose         2%
3. Peptone         2%

To prepare for 200ml media... Used 2ml Yeast Extract, 4ml Glucose, 4ml Peptone and 200ml distilled water... 

INGREDIENT USE FOR THE MEDIA...

PREPARATION TO MAKE MEDIA...

WEIGHING THE INGREDIENTS USED...

We weighed all the ingredients used to make the media by using the analytical balance...

BEFORE AND AFTER MIXING...

Then, pour all the ingredients and distilled water and dissolved it using magnetic stirrer...

After mixing, we put the cotton into the mouth of the conical flask and wrapped it with aluminium foil... 

AUTOCLAVE...

Next we autoclave the media... 121 Degree Celsius within 15 min...

Why 15 min not more than 15 min???

Because if more than 15 min the glucose will caramelize...

What next after autoclaving???

INCUBATOR SHAKER...

Lastly, we put it in the incubator shaker 200rpm, 30 Degree Celsius to used it for inoculation process next day........