Day 2: Preparation of fermentation

The second day of our preparation for fermentation start with the innoculation 1 colony of S.cerevisiae into innoculation medium.The meduim then was incubated in incubated shaker for 30C ,200 rpm and 24 hour.But don't forget to strelize the working area with 70% lysol.

The fermentation medium was ready in incubator shaker to be incubated overnight

FIRSTLY, STERILE THE WORKING AREA.

STEP TO INOCULATE THE YEAST...

After that,we prepared the fermentation medium that is 1% yeast extract,2% peptone,2% glucose with addition of 1.5 L distilled water.The mixture need to be mix properly before was poured into the fermenter vessel.The fermenter vessel that had ready with the ferementation medium then will be put onto the minifors to made an calibration for pH 4 and pH 7 using a pH 4 and pH 7 buffer respectively..

STEP FOR MEDIA PREPARATION

For next preparation of fermentation,we had done a calibration of pH electrode.This is the process to made an calibartion for pH electrod:

1 ) the silicon that was cover the tip of the electrode was removed.
2 ) the calibration option was put at high pH that is 7
3 ) the pH 4 buffer was prepared for calibration before the tip of probe was immersed in pH 7 until the we got the stable reading to ensure we got the right calibration.While the pH probe was immersed,the temperature probe also was immersed to ensure we got the right pH because pH nad temperature was react relatively.
4 ) the probe that was used to calibrate ph 7 was wash with distilled water
5) the ph 4 buffer was prepared for next calibration that was pH 4 calibration.The same thing was done as we made a pH 7 calibration before.
 6) The electrode was placed back on the ferementer after the calibration done and the ENTER was press.

 The pH and temperature probes was immersed in buffer  for calibration

Beside pH probe,pO2 probe also was put together in our fermentor.In this step,we have to unscrewed the metal section that was at the bottom.This step was crucial because we need to ensure there was no electrolyte inside the catridge membrane.More than that,this company was also provide a top up for a liqiud electrolyte if the the electrolyte level was low before the vessel with a ready pH and pO2 probe was fit onto the fermentor

Next,we all had prepared an antifoam reagent by inserting the antifoam into 250 ml bottle.In this process we have to ensure the tip wasreally connected with the metal pipe on the head plate and also need to ensure the clamp between the botle was clamped off.

The last step in preparaion of fermentor was an autoclaving process.But,before the autoclaving process,we need to check wether all the equipment like inlet filter was already connected with sparger or not, innoculation port and sampling bottle.Besides that,all the part that have directly exposed to the environment need to be foiled with aluminium to ensure the fermentor was remain sterile even after taking it out from autoclave.

  The fermentor with all ready probes including antifoam was ready for autoclaving process.